Composite

Part:BBa_K4229016:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)


RBS-snoopCatcherTnaA-RBS-FRE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 990
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 990
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 990
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 990
    Illegal AgeI site found at 155
    Illegal AgeI site found at 241
    Illegal AgeI site found at 1613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2150
    Illegal SapI.rc site found at 2270


Design Notes

none


Source

e.Coli (MG1655 strain). This biobrick itself was synthetised. The use was taken out of a paper.

References

Yin H. et.al., "Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI in Escherichia coli", ACS OMEGA, vol.6, 20569−20576, 2021, https://doi.org/10.1021/acsomega.1c02679.